The effect of quercetine on lipid peroxidation induced by ziprasidone in human plasma – in vitro studies

Zakład Psychiatrii Biologicznej Międzywydziałowej, Katedra Fizjologii Doświadczalnej i Klinicznej, Uniwersytet Medyczny w Łodzi
Correspondence to: Dr hab. n. med. Anna Dietrich-Muszalska – kierownik Zakładu Psychiatrii Biologicznej Międzywydziałowej Katedry
Fizjologii Doświadczalnej i Klinicznej Uniwersytetu Medycznego w Łodzi, ul. Mazowiecka 6/8, 92-215 Łódź, tel.: 42 272 56 59, faks: 42 272 56 52, e-mail: tzn_lodz@post.pl
The project has been financed by the means of the University of Łódź, research No 502-03/1-155-02/502-14-106

PSYCHIATR. PSYCHOL. KLIN. 2014, 14 (1), p. 10–19
DOI: 10.15557/PiPK.2014.0001
STRESZCZENIE

Some antipsychotics, including ziprasidone (ZIP), contribute to pro- and antioxidative imbalance in schizophrenic patients. Therefore, searching for effective antioxidative supplementation decreasing antipsychotics prooxidative effects has a high clinical importance. The aim of the study was to establish the effect of ZIP on human plasma – by determining the levels of thiobarbituric acid reactive substances (TBARS), in in vitro model. Material and methods: Blood samples were obtained from healthy male volunteers and placed in the ACD solution. The active substance, i.e. ZIP, was dissolved in 0.01% solution of dimethylsulfoxide to reach the final concentrations (40 ng/ml, 139 ng/ml) and incubated with plasma (for 1 and 24 hours at 37°C). Plasma was also incubated with quercetine (7.5 μg/ml, 15 μg/ml) and with quercetine and ZIP, in different combinations of tested concentrations. Control samples (without the drug) were performed for each experiment. TBARS concentrations were determined using Rice-Evansspectrophotometric method (modified by Wachowicz and Kustroń). Results: ZIP at the concentrations of 40 ng/ml and 139 ng/ml after 24 hours of incubation with plasma causes an increase in TBARS (p respectively <0.01 and <0.002). Quercetine (7.5 μg/ml, 15 μg/ml) incubated for 24 hours in plasma with ZIP decreases lipid peroxidation on average by 38% (for ZIP 40 ng/ml p respectively <0.0003 and <0.0001, for ZIP 139 ng/ml p respectively <0.002 and <0.004). Conclusions: Quercetine significantly decreases lipid peroxidation induced by ziprasidone.

Słowa kluczowe: antipsychotics, ziprasidone, quercetine, lipid peroxidation, schizophrenia